Electrify Your Structural Variant Analysis
Are you a human genomics researcher on a quest for precise, cost-effective structural variant (SV) analysis in your field of study? Nabsys, in collaboration with Hitachi High-Tech America, presents the opportunity to harness the power of high-resolution SV analysis with electronic genome mapping (EGM) to get insights unachievable by conventional technologies.
For a limited time, apply for the OhmX RAMP UP Grant for free access to the OhmX platform.
How does EGM work?
Watch this video to learn how electronic genome mapping works, including the step-by-step process of identifying structural variants, as well as the comprehensive bioinformatic analysis.
The laboratory workhorse for both structural variant verification and whole genome structural variant discovery
Utilizing state-of-the-art electronic nanodetectors, the OhmX platform detects tagged high molecular weight DNA molecules with unprecedented precision and speed offering a superior and reliable tool for structural variant analysis.
Submit your application today!
By submitting your application to the OhmX RAMP UP Grant, you will be eligible to receive:
No Instrument Cost
Receive installation of 1 to 5 OhmX systems at no cost, tailored to your research needs and utilization
Starter Kit Supplied
Access to a starter kit with essential consumables to ensure smooth operation of the OhmX system during initial runs
Bioinformatics Suite
Complimentary access to Human Chromosome Explorer (HCE) software for structural variant analysis in Google Cloud
Premium Support
Benefit from dedicated field application support from Nabsys to optimize instrument use and achieve research goals
Democratization of structural variant analysis
The OhmX Platform was designed to maximize accuracy, while minimizing cost, ensuring scalability in your human genomics research.
Application Process
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Initial Discussion
Candidates should engage in a preliminary discussion with a Nabsys representative to understand program details and requirements. View official grant rules here.
Grant Application Submission
Using 350 words or fewer, submit a proposal for a genomics research project relevant to human structural variant analysis. Tell us how you will accelerate your science with the OhmX platform.
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Application Review
Nabsys reviews applications on a rolling basis, evaluating:
- Sample type and quality
- Proposed structural variant analyses
- Anticipated utilization of the OhmX system
- Publication goals
- Supported location
Project Approval
If selected, grant awardees will sign an optional Non-Disclosure Agreement (NDA) and a Consignment Agreement with Hitachi High-Tech American for the OhmX Analyzer. A training and installation plan will be determined following the signed agreements, so your experiments can begin!
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Leverage EGM to drive your publications!
Apply today for the chance to use electronic genome mapping in your research
Resources
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OhmX Product Brochure
Overview of electronic genome mapping, how the technology works, product features, the bioinformatics and reporting pipeline, and key application areas.
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Boston Children's Hospital Case Study
Orthogonal verification of a tandem duplication suggested by short-read NGS with duplication breakpoints later estimated by optical genome mapping.
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OhmX Sample Guide
Guidance on current supported sample types and the preferred method for collecting and storing DNA for the OhmX platform.
Frequently asked questions
How does electronic detection work?
When DNA enters the nanochannel, it blocks the current that can go through the channel and is measured as a voltage change. When a tag is also present on the DNA, the current is further reduced resulting in a sharp signal. The voltage is measured as a function of time so the time that a channel is empty, the time it is occupied by untagged DNA, and the time each tag goes through the channel can be determined. These times are converted to distances for each molecule.
How does EGM differ from OGM?
Both EGM and OGM place tags on isolated high molecular weight DNA according to known locations in the genome and then use the distances between tags to align sequence reads to each other or to the genome. EGM and OGM differ in how those tags are applied to the DNA and how they are detected.
EGM: uses voltage differences induced by current blockade to locate the tags with high resolution.
OGM: uses fluorescently-labeled tags that are excited by lasers and then detected with high resolution imaging systems. The use of light introduces both expense (camera and lasers) and resolution limitations due to the diffraction limits of light and the impact that has on resolving labels that are close together.
Why is EGM resolution so much better than other technologies?
Optical imaging is reliant on the wavelengths of light to detect tags on DNA molecules. The wavelength of light limits the separation at which different tags can be distinguished and increases the noise in the tag positioning. This limits the resolution of any optically-based system. EGM relies on measuring voltage change as DNA moves through a nanochannel which can be done quickly and with higher resolution.
How much coverage is expected to assemble a human genome?
At present, about 150x raw coverage is required to enable de novo assembly. We are expecting coverage requirements to decrease as we optimize workflows.
What is the throughput per year for a single OhmX Analyzer?
Maximum throughput is 3 samples per week, 150 genomes per year for full utilization for one instrument.
Who is this grant available for?
The OhmX for SV Analysis Grant is available to any qualifying human genomics researcher residing in the U.S.
Are reagents included in the grant?
Yes - a start kit of reagents is included with the OhmX Analyzer, however, grant recipients are responsible for purchasing further reagents beyond the starter kit.